Sentence examples for both cell lines including from inspiring English sources
Searching for genes affected led to the identification of multiple genes repressed by CBX6's overexpression in both cell lines, including bone marrow stromal cell antigen 2 (BST2).
The derivation of EPT1 from EP156T was clearly verified by the similar expression levels of hTERT, the puromycin resistance and the very similar karyotypes of both cell lines, including a common derivative chromosome, der(20).
A number of genes involved in cell cycle regulation and proliferation were modulated in both cell lines, including CDKN1A and MAK.
Of these differentially expressed genes, 68 genes (39 62%) were commonly down- or up-regulated in both cell lines including RPS6KB1, ABL1, PPP1R12B, PRKCQ, and STK32B [see Additional File 1].
Expression analysis showed an expected profile of gene expression in both cell lines, including known TGF-β targets (Additional file 7: Table S4, Additional file 8: Figure S4c for qRT-PCR validations).
A number of genes involved in xenobiotic metabolism and transcriptionally activated via the aryl hydrocarbon receptor (AHR) were up-regulated in both cell lines including NQO1, AKR1C3, AKR1C1, MGST1 and CPM.
The up-regulated pathways common to both cell lines include the metabolism-related such as steroids, pyruvate, glycolysis glutathione, or glycerolipid, and not directly related to the control of cellular proliferation.
In addition to genes with diverse functions that are not directly related to HH-dependent proliferation, up-regulated genes that influence the G1/S transition and subsequent cell cycle progression, and that are common to both cell lines, include CDKN1A, and the DNA-damage-inducible transcripts 3 and 4 (DDIT3 and DDIT4).
Over-represented themes among the up-regulated genes in both cell lines included oncogenesis and cell cycle progression from G2 to M phase.
Expression changes observed in both cell lines included genes involved in xenobiotic metabolism (e.g., CYP1B1, NQO1, MGST1, AKR1C1, AKR1C3, CPM), cell cycle regulation (e.g., CDKN1A), apoptosis/anti-apoptosis (e.g., BAX, IER3), chromatin assembly (e.g., histone genes), and oxidative stress response (e.g., TXNRD1).
