Both cell lines grow as adherent monolayer.
Greater potentiation was observed in both cell lines growing anchorage dependently compared to anchorage independently.
From this data, the value of the sustained Qp phenotype is clear, i.e. in Cell Line Pair A where the initial Qp for both cell lines was similar and both cell lines grew to comparable VCDs, the SQp cell line generated 1.8-fold more protein over the duration of the culture through maintaining its Qp.
While nucARRB1 cells grew significantly faster than control cells in glucose-containing medium, both cell lines grew at the same slower rate in galactose-supplemented medium, implying that nucARRB1 cells rely on glucose catabolism to sustain their increased proliferation rate (Fig 7F).
Both cells lines grew in Dulbecco's Modified Eagle Medium, 2 mM l-glutamine, 100 μg/mL streptomycin, 100 units/mL penicillin, and 10% foetal calf serum (FCS).
To define the mechanism of lumen formation in prostate acini we examined both theories in several cell lines grown in 3D Matrigel culture.
As in monolayer cultures, resveratrol significantly increased levels of p53 in both of these wt p53 cell lines grown as spheroids (A375, P = 0.0270; YUZAZ6, P = 0.0422).
All three cell lines grown in both serum and serum supplemented with neurotrophic factors expressed NF-M, though there was more evidence of neuronal-like morphologic changes in the supplemented cultures (Fig. 3, top row, best seen in IMR-32).
Principal component analysis (PCA) was performed on the projected J-RES NMR data sets of the two untreated cell lines grown in both hypoxic and normal conditions; the resulting PCA scores plot (Supplementary Figure 1) highlights remarkable differences between the metabolomes of K562 and KG1a cells (almost 70% of the variability is captured by the first principal component).
