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Both cell lines formed tumours at the site of injection.
Both cell lines formed neurospheres when cultured in CSC medium.
When cultured in agarose gels, both cell lines formed spheroids.
Both cell lines formed mammospheres within 3-5 days, reaching ~50 μm in diameter.
Both cell lines formed five subcutaneous tumors out of a total of five injected sites.
Both cell lines formed amorphous clusters in sharp contrast to the well-organised-appearing formations of control cell lines.
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When inoculated into the mammary fat pad of athymic mice, both cell lines form primary tumors.
When orthotopically inoculated into athymic mice, both cell lines form primary tumors, but only M4A4 is capable of metastasis to the lungs and lymph nodes [ 2, 3].
To study the impact of increased MT1-MMP expression on cyst enlargement, we compared the growth of the cysts formed by parental MDCK cells to that of the cysts formed by MDCK-MT1-MMP cells in 3D gels with a collagen concentration of 2.5 mg/ml, at which both cell lines form cysts predominantly.
Cell lines formed two groups, according to the presence of senescent cells in isolated colonies.
All cell lines formed spheroids that could self-renew and be serially re-passaged.
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