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Strikingly, whereas P3H1 was present in all cell lines, expression of both P3H2 and P3H3 was undetectable in several lines at both mRNA and protein levels.
Cell lines, expression analysis and chromatin immunoprecipitation.
Cell lines, expression constructs, RNAi and antibodies.
When we knocked down TPM3 in HepG2 and SNU-475, the expression levels of Snail and E-cadherin became reversed in both cell lines; Snail expression was significantly decreased and E-cadherin was significantly increased compared with the siNEG transfection control on both mRNA and protein levels.
The results showed that eIF5A-2 was expressed in the control cells of both cell lines; however expression was higher in NCI-H1299 comparedmpared to A549 cells.
In both cell lines, the expression of the three D-type cyclins increased as a result of induced proliferation, whereas the expression of cyclin D3 increased as a result of induced differentiation.
A basal expression of bone-related genes in favour of an osteoblastic response was detected in both cell lines, while expression of genes associated with osteolytic events and osteoclastogenesis was low or undetectable.
In both cell lines, the expression of Cdc2 and cyclin B1 was strongly decreased 72 h after transfection.
In both cell lines, the expression of the hTERT gene was markedly downregulated as a result of bortezomib treatment.
In both cell lines, the expression level of mdr-1 and MRP genes was upregulated after both survival and regrowth in the anti-cancer drugs.
In both cell lines, MMP7 expression was significantly downregulated; levels were similar to that of MMP7-specific siRNA treated cells (Fig. 3a).
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