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In contrast, both cell lines expressing K49R-mutated MSRA kept the resistance even with ARD1 coexpression.
A decreased ability to invade was observed for both cell lines expressing SUSD4 as compared to mock-transfected cells (Fig. 3e, j).
In a comparative microarray analysis, we were struck by the predominance of upregulated proneural genes in both cell lines expressing SYT-SSX2 [ 26].
However, both cell lines expressing either mutant PIK3CA isoform showed a significant increase in the IC50 for both Akt and S6 phosphorylation.
The two endogenous proteins readily and specifically co-immunoprecipitated in both cell lines expressing the mutant forms of p53 (Fig 5A).
Trypan blue analysis of the number of viable cells showed a significant anti-proliferative effect in both cell lines expressing hHSS1 as compared to the control cells (A172/U87 wild-type and A172/U87-pcDNA3.1 empty vector).
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As observed in Figure 3A both cell lines express VEGFC, however, higher expression in metastatic IIB-BR-G-MTS6 tumors was found as compared with IIB-BR-G in vivo.
The prostasin gene promoter is minimally heterogeneously methylated in the UM-UC-9 and unmethylated in the UM-UC-5 at a site critical for prostasin expression and both cell lines express a high level of prostasin [ 9].
Both cell lines expressed low levels of MHC class I and no expression of MHC class II on the cell surface (Figure 1A).
Both cell lines expressed COX-2, although MDA-MB-468 cells exhibited lower protein expression than did MDA-MB-231 cells.
Measurements of mRNA expression of the EGF family of ligands showed that both cell lines expressed high levels of AREG and modest amounts of HB-EGF and TGF- α.
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