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As observed in Figure 3A both cell lines express VEGFC, however, higher expression in metastatic IIB-BR-G-MTS6 tumors was found as compared with IIB-BR-G in vivo.
The prostasin gene promoter is minimally heterogeneously methylated in the UM-UC-9 and unmethylated in the UM-UC-5 at a site critical for prostasin expression and both cell lines express a high level of prostasin [ 9].
Importantly, both cell lines express LRP-1.
Both cell lines express CCR4 and migrate in response to CCR4 ligands.
These data support that both cell lines express functional p53 and phosphorylation of ATM/ATR substrates such as p53 and H2AX, in response to DNA damage.
The reason for the unresponsiveness of Jurkat E6.1 and HuT78 T cells to fibronectin is unknown, since both cell lines express VLA-4 on their surface.
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Both cell lines expressed low levels of MHC class I and no expression of MHC class II on the cell surface (Figure 1A).
Both cell lines expressed COX-2, although MDA-MB-468 cells exhibited lower protein expression than did MDA-MB-231 cells.
Measurements of mRNA expression of the EGF family of ligands showed that both cell lines expressed high levels of AREG and modest amounts of HB-EGF and TGF- α.
Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that both cell lines expressed a range of β-cell transcription factors and pancreatic hormones.
Both cell lines expressed C2GnT1 and FucT-III mRNA necessary for C2-O-sLeX synthesis (Fig. 2A).
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