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Both cell lines exhibited biallelic expression, suggesting loss of imprinting.
Both cell lines exhibited ∼90% confluence by the time of assay.
Moreover, EVs from both cell lines exhibited a structure that is sealed by tight junctions (Figure 7b in A and B).
At post confluence (day 0) both cell lines exhibited fibroblast-like morphology and expressed the SV40 TAg, as demonstrated by its nuclear immunodetection (inset).
As shown in Fig. 11, by day 7, both cell lines exhibited a significant increase (p<0.01) that averaged 1.4-fold in cell number in response to sCXCL16 addition.
Finally, both cell lines exhibited lower amounts of VEGF secretion in the presence of IL-2 treated NK cells, indicating the ability of IL-2 treated NK cells to exert significant inhibitory effect on VEGF secretion.
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Both cell lines exhibit comparable growth rate with the doubling time about 24 h.
In separate experiments, treatment with medroxyprogesterone 17-acetate (MPA) showed no effect on cell viability, confirming that both cell lines exhibit resistance to hormone treatment.
Next, we asked if both cell lines exhibit common temporal expression profiles rather than showing different effects for the same set of genes.
However, it is also apparent that both cell lines exhibit increased cyclin B1 expression at doses of taxol that did not induce a measurable G2/M arrest on the basis of single parameter DNA measurements.
At 9 h following addition of taxol both cell lines exhibit an accumulation of cells in G2/M, this accumulation being more pronounced in the DoHH2 cell line, with 43% of the cells being in G2/M as opposed to 30% of the SU-DHL-4 line.
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