The results demonstrated the concurrent expression of both Mcl-1 and Bcl-xL in most cell lines, corroborating the immunostaining results in both lung and colon tumor tissues shown in Figure 1.
The treatment of cells with purified anthocyanin extract or crude extracts at 5 800 μg mL−1 did not show significant cytotoxic effects on the cell lines, corroborating the chemical antioxidant effect of the extracts (DPPH assay).
Many small RNAs in both orientations were detected, including some that were specific to one or two of the cell lines corroborating results described previously (Supporting Information, Figure S1A and B).
Our findings clearly show that the selection of an appropriate tissue culture insert is critical when establishing a BBB model using these immortalized human brain capillary endothelial cell lines, corroborating previous findings in which a substantial impact of material characteristics on the adherence of cells and barrier tightness was demonstrated [ 31, 32].
The observations on cell lines corroborated the results of routine histological examination of PDT-treated B16 melanoma and M-1 sarcoma in which massive necrotic zones are detectable as early as 24 h post PDT (not shown).
Moreover, transient up-regulation of Hmox1 in all tested cell lines corroborates with the inhibitory effects of mastic oil on tumor-related inflammatory response [ 17].
Our results showing that the U373 cell line, which expresses low levels of RB, is more sensitive to VP-16 treatment compared with the other cell lines corroborates this possibility.
In this sense, in the present study, the inhibition of HOXB7 expression in MIA PaCa-2 and Capan-1 cell lines corroborated the participation of this homeobox gene in the development of PDAC, reinforcing the need for further investigation.
Albeit MMP-1, -2 and -13 did not stain with either the MDA-MB cell line or with control cells, immunocytochemistry of the MDA-MB-468 cell line corroborates the results obtained by Western blot analysis.
These observations corroborated the results obtained during the generation of both cell lines (H 6-GFP-BD-CVIL and SV40-mRFP/H 6-GFP-BD-CVIL) during the study presented here.
