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In fact similar effects could be demonstrated in both cell lines confirming the results of Colo-357.
M-CM culturing led to an approximately 2-fold increase in β-catenin dependent luciferase activity in both cell lines, confirming that β-catenin became transcriptionally activated upon M-CM treatment.
CoCl2-induced hypoxia significantly enhanced doxorubicin resistance, whereas inhibition of HIF-1α fully abrogated this resistance in both cell lines, confirming that HIF-1α is necessary for CoCl2-induced doxorubicin resistance in these cells.
Finally, anti-H MAbs (3900 and 1347 [ 26]) neutralized both viruses in both cell lines, confirming that H was anchored in both virus' envelopes and that F alone was not sufficient to support viral cell entry in these cells (data not shown).
Similarly in LNCaP and PC-3 prostate cancer cells, classified respectively as androgen-sensitive and androgen-insensitive, MF was efficacious in growth-inhibiting both cell lines, confirming previous data using androgen-refractory LNCaP cell variants in vitro [ 8, 12] and in vivo [ 12, 39].
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However β-catenin reduction in both cell lines confirmed that indeed the canonical WNT pathway regulates ABCB1 expression as shown by 60%and71%1% reduction of ABCB1 mRNA levels in K562 and Lucena cells respectively.
On the other hand, iSHMT2 induces a significantly lower-apoptotic effect on both the cell lines confirming that, unlike other cancer cell types, SHMT1 has a more important role than SHMT2 in lung cancer cell survival.
In line with the use of pathway inhibitors to decrease pCREB, CREB silencing via shRNA resulted in upregulation of AP-2α expression in both metastatic melanoma cell lines, confirming that CREB indeed acts as a negative regulator of AP-2α (Figure 3A).
However, our immunoprecipitation experiments show that p53 directly interacts with BRD4 in both the OCI-AML3 and Hela cell lines confirming the recently published observations of Wu et al. [ 29].
Again, we extended this analysis to the other cell lines, confirming that CSB suppression dramatically enhanced apoptosis in tumor but not in non-tumoral cell lines (Fig. 4C).
The band of 670 bp was also present in the Tko-II and Tko-III cell lines confirming the gene-deficient status of these cells.
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