In fact similar effects could be demonstrated in both cell lines confirming the results of Colo-357.
CoCl2-induced hypoxia significantly enhanced doxorubicin resistance, whereas inhibition of HIF-1α fully abrogated this resistance in both cell lines, confirming that HIF-1α is necessary for CoCl2-induced doxorubicin resistance in these cells.
M-CM culturing led to an approximately 2-fold increase in β-catenin dependent luciferase activity in both cell lines, confirming that β-catenin became transcriptionally activated upon M-CM treatment.
Finally, anti-H MAbs (3900 and 1347 [ 26]) neutralized both viruses in both cell lines, confirming that H was anchored in both virus' envelopes and that F alone was not sufficient to support viral cell entry in these cells (data not shown).
Similarly in LNCaP and PC-3 prostate cancer cells, classified respectively as androgen-sensitive and androgen-insensitive, MF was efficacious in growth-inhibiting both cell lines, confirming previous data using androgen-refractory LNCaP cell variants in vitro [ 8, 12] and in vivo [ 12, 39].
The analysis of the expression pattern of these transcription factors in both human liposarcoma cell lines confirmed an expression pattern similar to that previously observed in liposarcomas derived from FUS-DDIT3 transgenic mice (Figure 1B-C), although we detected variable, although low, levels of PPARγ1 in the human liposarcoma cell lines.
In addition, our detailed functional assays conducted in both endothelial and nonendothelial cell lines confirmed and strengthened the associations of the HSPA8 gene variations and CHD.
Western blot analysis of isolated nuclear and cytoplasmic fractions from both canine and human OSA cell lines confirmed enrichment of the 30 kD HES-1 protein in the nuclear fraction while the non-specific bands were enriched in the cytoplasm fraction.
Furthermore, RT-PCR analysis of TSC1and totaln total RNA from 8 clear-cell RCC cell lines confirmed expression of both TSC genes.
In vitro cell proliferation assay and morphological analysis of mouse fibroblast L929 cell lines confirmed the biocompatibility of CS-keel.
