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LD caused significant genotoxic damage to both cell lines compared to their respective controls.
The result demonstrated a significant reduction of cell viability in cells cultured in 8 μM SD for 6 days from both cell lines compared to that of the controls (Fig. 1).
Following PAF stimulation for 1 h, nuclear translocation of STAT3 was observed in both cell lines compared with unstimulated controls (Figure 6 a j).
As expected, the unencapsulated mutant B5055nm showed increased cell association (greater than 10-fold) to both cell lines compared with the wild type capsulated strain, confirming previous findings that the capsule reduces bacterial adherence [31], [33] (Fig. 2a and b).
ER-βmRNA levels are distinctly lower in both cell lines compared with ER-α.
Snail and SIP1 transfectants showed undetectable expression of E-cad mRNA in both cell lines, compared with the control transfectant.
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Truncating a portion of the promoter between -594 and -283 resulted in increased luciferase activity in both cell lines (compare CbxD98 and CbxU283 in Figure 2a).
When cross-referenced with results reported in Figure 4, nine of these putative target transcripts were found to be up-regulated in both melanoma cell lines compared to those in melanocytes and down-regulated in both melanoma cell lines when miR-211 was stably expressed.
Results show that miR-211 is elevated in both melanocyte cell lines compared to normal human skin (Figure S1).
DNA Pol α and Cdc45 were highly enriched in both transfected cell lines compared to control cells at each of the time points.
The growth curves obtained (Figure 1C) demonstrated that the cell growth was reproducibly reduced in both shHP1γ cell lines compared to control ESC.
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