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In both cell lines, cells accumulated in G1, 24 hr after treatment with GANT61.
A less marked increase in histone H4 acetylation was observed in both cell lines cells (Figure 3A and B).
Both cell lines cells are derived from a human malignant glioma / grade III tumor.
For both cell lines, cells were fixed with ice cold methanol: acetone (50 50) for 15 min at 4°C.
In both cell lines, cells without Tualang honey treatment showed polygonal shape, which is considered as the normal cell growth phenomenon.
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Interestingly, it was observed that, in both cell lines, cell proliferation rate increased (approximately 3 fold) whenever extracellular Ca2+ was increased (2.5 mM, 2.5 mM+R-467 and 2.5 mM+2.50+R-467).
In both cell lines, cell growth/proliferation was stimulated by Ca2+ or calcimimetic NPS R-467 addition and their effects were reversed by the CaSR antagonist NPS 2390 even if the two cell lines behaved differently.
Moreover, in both cell lines, cell-cycle alterations correlated with an increase in cyclin E expression, which typically reached a peak at the G1 S transition.
For both HeLa cell lines, cells were grown and maintained in DMEM medium (Nissui Pharmaceutical Co., Tokyo) supplemented with 10% fetal calf serum and incubated at 37°C in 5% CO2 with constant humidity.
After Yang et al. [89] exposed SK-N-SH (human neuroblastoma cell line) and neuro2a (mouse neuroblastoma cell line) cells to silica NPs, they found that ROS production was enhanced and cell viability reduced in both cell lines.
Staurosporine (STS) induced cell death in both cell lines.
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