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Increasing curvature resulted in higher expression levels of tight junction protein occludin in both cell lines, but did not induce a change in expression of adherens junction protein E-cadherin.
Treatment with NSAIDs or gemcitabine altered the cell cycle phase distribution as well as the expression of multiple cell cycle regulatory proteins in both cell lines, but did not induce substantial levels of apoptosis.
For [125I]SGMIB-MNT, the relationship between clonogenic survival and radioactivity concentration added to the medium was poorly fitted by a one-exponential model for both cell lines but were fitted effectively with two-exponential equations.
As expected, MG132 was toxic on both cell lines, but was significantly more toxic in G93A SOD1 expressing cells.
Upon reoxygenation (ambient O2), HIF-1α levels decreased in both cell lines but in COMMD1 knockdown cells the rate of HIF-1α degradation was markedly reduced.
The expression of NLS-p65 was similar in both cell lines, but under oxaliplatin treatment its activation was higher in the resistant cell line (Figure 6C).
Three ligands, AREG, HB-EGF and TGFA, activated EGFR even in the presence of cetuximab in both cell lines, but the effects of EGF on EGFR in the presence of cetuximab were minimal.
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Both DAC and DAC + SAHA treatments induced ROS generation in both cell lines tested, but this effect was more pronounced in HL-60 cell line.
The same trends were observed for both cell lines studied but with considerably greater concentration being obtained for P4E6 as compared with OnyCap23 cells.
In addition, we found that both cell lines express hSpred1 but not hSpred2 protein (Fig. 3A); curiously hSpred1 protein was detected as a double band, probably due to post-translational modifications [6], [19].
We consider both cell lines as transformed but non tumorigenic.
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Justyna Jupowicz-Kozak
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