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Notably, both cell lines are globally methylated unlike our DKOzero line and therefore make it more difficult to determine the exact scale of the off-target activity.
Both cell lines are invasive ductal carcinomas and thus represent one of the most commonly encountered types of cancers.
This also means that both cell lines are derived from the same tissue which helps limit the potential variations between tissue types.
Both cell lines are immortalized, yet non-oncogenic in athymic mice.
Representative observations from multiple experiments with both cell lines are presented in Fig 8.
Copy number results for both cell lines are attached as Dataset S1 and Dataset S2.
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Both cell lines were tested for mycoplasma contamination using a PCR-based method.
Both cell lines were incubated with Sgc8-FSNPs.
Both cell lines were deficient in the human mismatch protein MutLalpha.
Both cell lines were incubated at 37 ºC, 5%% CO2, and saturate humidity.
Both cell lines were a generous gift from Professor Karl-Norbert Klotz (University of Würzburg, Germany) [40].
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