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Both cell lines appeared to express the relevant ORs at comparable levels (Figure 5D).
Both cell lines appeared to display hypomethylated ABCB1 DNA.
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In particular, we detected two distinct populations, one consisting of only CD133+ cells and the other consisting of only CD133− cells, in DLD-1 cells, while the other cell lines appeared to possess one population consisting of both CD133+ and CD133− cells.
Colonies generated from clonal growth of Oct4λVP2∗ cell lines appeared similar to wild-type Oct4-supported cell lines, both forming differentiated cells at the periphery and both sensitive to the differentiation-induced effect of Oct4 overexpression.
The differences in drug sensitivity reported here among the various cell lines appeared to be unrelated to drug transport, in terms of both influx and efflux.
We also noted that many cell lines appeared to be heterogeneous with respect to PHD3 promoter methylation status.
Several cell lines appeared to exhibit a high frequency of SNPs in the region −239 to −228.
As Oct4λVP2 and Xlpou91λVP2- expressing cell lines appeared identical, we focused on Oct4λVP2-supported cell lines.
Under general cell-culture conditions, growth kinetics of all three cell lines appeared similar.
After 60 passages, the cell lines appeared homogeneous and no fibroblast like cells could be detected.
The nature of the reduced survival induced by FAC in the sensitive cell lines appeared diverse.
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