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RNA was collected from each cell lineage and hRasGRP4 expression was examined for both cell lineages using TaqMan MGB probes specific for hRasGRP4 and hGAPDH.
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Although many studies have established techniques to successfully differentiate stem cells into different mature cell lineages using growth factors or extracellular matrix protein supplementation in both two and three-dimensional configurations, they are often limited by lack of control and low yields of differentiated cells.
A reasonable strategy is to mimic the stem cell microenvironment for the differentiation of hPSCs into specific cell lineages using optimal polymeric biomaterials for hPSC culture.
Furthermore, our results are coincident with the results obtained on erythroid cell lineages using the same metrics [20].
Total RNA was extracted from the different cell lineages using the Trizol reagent according to the manufacturer's instruction (Invitrogen).
Immunophenotyping was performed on frozen vastus lateralis muscle biopsy sections to determine infiltrating cell lineages using established monoclonal antibodies to canine lymphocyte antigens [ 39].
Although attempts have been made to relate some embryonic and postembryonic cell lineages using the morphology of characteristic embryonic cells and marker gene expression in late larvae (Truman et al., 2004), the existing evidence is rather indirect and incomplete.
To validate our findings from gene expression microarrays, we examined mouse medulloblastomas for markers of cell lineage using IHC.
We investigated the functional significance of these domains in the ureteric cell lineage using genetic murine models of deficient or constitutively active HH signaling.
Since endogenous gata-1 expression was found to be unchanged in our zTERT morphants (Figure 3B, g, h), it was possible to trace the erythroid cell lineage using gata-1GFP-transgenic gata-1GFP-transgenic gata-1GFP-transgenic
In this study, they targeted expression of I-Eα self-antigen to the B cell lineage using the human CD19 promoter and studied negative selection in the I-E negative C57Bl/6 background.
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