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Both cell cycle distribution and apoptotic cells proportion were then analyzed with a BD FACSCanto II flow cytometer (BD Biosciences) and FCS Express 4 (BD Biosciences).

Both cell cycle distribution and the cell surface E-cadherin expression of the three cell lines were analyzed using a Coulter EPICS™ XL-MCL flow cytometer (Beckman-Coulter, Fullerton, CA, USA) equipped with a 15 mW at 488 nm argon ion laser as the excitation source.

Both cell-cycle distribution and expression of cyclins were not affected by RTX or GA101 treatment.

To elucidate the mechanism of action, we further examined effects of both drugs on cell cycle distribution of the cells in combination and/or alone.

For flow cytometric analysis of cell cycle distribution, both adherent cells and cells in the culture media were harvested by centrifugation, resuspended in 70% ethanol (−20°C) and stored at −20°C.

Cell cycle distribution percentages were calculated using Cell Quest software (Becton Dickinson).

Both the growth rate and the cell cycle distribution of cultured GmnnΔ/Δ neurosphere cells are indistinguishable from controls.

The cell cycle distribution for both cell lines (Cal-78 and SW-1353) isummarizeded in form of the stacked bar chart given in Fig.  2a.

To evaluate the mechanism underlying shNUF2-mediated cell growth inhibition, the cell cycle distribution in both PANC-1 and Sw1990 cell lines was detected by flow cytometry.

Cell cycle distribution of both adherent and sphere-forming cells was analyzed by flow cytometry at 48 h after treatment with chemotherapeutic agents and irradiation.

Cell cycle distribution was measured both in the untreated and paclitaxel treated cells.