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To identify factors selectively secreted by G2B-10A oR2-T1ASAS cells, we screened conditioned medium derived from both cell cultures, using a cytokine antibody array.
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Briefly, total DNA (both mitochondrial and genomic) was extracted from A549 or A549 ρ0 cell cultures using the QIAamp DNA Mini Kit (Qiagen).
The genomic DNAs were extracted from D. vulgaris Hildenborough and D. desulfuricans cell cultures using Blood & Cell Culture DNA Kit (Qiagen, Hilden, Germany).
Similarly, Li et al. [1] successfully enhanced the diosgenin production in Dioscorea zingiberensis cell cultures using endophytic fungal infection.
Exosomes have been purified from biological fluids and in vitro cell cultures using a variety of strategies and techniques.
RNA was prepared from primary myoblast or C2C12 cell cultures using Trizol reagent (Gibco).
Total RNA was isolated from cell cultures using TRI reagent (Sigma-Aldrich, Munich, Germany).
Cell viability was examined in separate T cell cultures using trypan blue (2.5%) at 64 h of incubation.
RNA was harvested from cell cultures using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's suggested protocol.
Several attempts have been made to produce cell cultures using either fetal CP or CP tumors.
Total RNA was extracted from paired tissues and cell cultures using TRIzol (Invitrogen).
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