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Both cell cultures treated or not treated with VPA were incubated with 1% of periodic acid (Sigma-Aldrich) in acetic acid for 30 min.
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Glioblastoma cell photoinactivation was observed in cell cultures treated with ZnPc as well as with TAZnPc.
Genomic DNA was extracted from these and from parallel cell cultures treated in the absence of topotecan, and DNA libraries were prepared and sequenced.
Suboptimally transduced T cell cultures treated with O6-benzylguanine and BCNU were selected from 3 to 100%, and after selection cultures did not support HIV replication.
Primary bovine muscle cell cultures treated with the β-adrenergic agonist treatment in DMEM media had greater protein degradation than did controls in DMEM (P < 0.05).
Infected cell cultures treated with medium containing 20 μg/ml tetracycline proved to be non-infective when inoculated into susceptible splenectomized calves.
However, primary bovine muscle cell cultures treated with the β-adrenergic agonist treatment in SKBM media had reduced protein degradation than did controls in SKBM (P < 0.05).
The potential protective activity of the new compounds was evaluated using nitric oxide (NO) production in BV2 microglial cell cultures treated with lipopolysaccharide (LPS), and the toxicity of the new compounds tested using a cell viability assay.
In a study led by David Schaffer and Adam Arkin of the University of California, Berkeley, around 80% of latent HIV became active in cell cultures treated with a combination of SAHA and prostratin.
Cell cultures treated in this manner were harvested by centrifugation at 5,000 × g for 10 min at room temperature and washed with 50 mM EDTA (twice) followed by resuspension in EC buffer (6 mM Tris, 1 M sodium chloride, 100 mM EDTA, 0.5% w/v Brij58, 0.2% w/v sodium deoxycholate and 0.5% v/v sarkosyl, pH 7.5).
Lower cytotoxicity was observed at a dose of 6.25 μg/mL in cell cultures treated with this graphene oxide.
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