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In both cases the template cDNA is scarce unless it is cloned in a vector and amplified in E. coli cells.
In both cases, the template has to be pre-fitted in the density map, or at least placed in an approximated position.
Similar(58)
In both cases since the template PDB originally had segments of rRNA attached to it, the homology modeling yielded a 'target' conformation of GlSec61α resembling the ribosome-complexed state (Fig. 1b, Panels i and iv).
In such cases, the template prediction for the flanking TMHs should be used to guide the loop template selection.
In some cases, the template may be selected by the triage staff or based on triage staff documentation.
In this case, the template inherits the vocabulary, constraints, resources, and relationships defined in the extended template.
(MCDA as a technique does not elicit the inputs into it, but in this case the template was used as the preference-eliciting device).
This is similar to an inverse PCR strategy, although in this case the template is unmodified genomic DNA.
In each case, the template contained at least 500 bp of coding sequence.
If this is the case, the template concentration has to be checked and the dilution series have to be redone.
There may be compounds for which this is different, in which case the template algorithm needs adjusting.
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CEO of Professional Science Editing for Scientists @ prosciediting.com