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In both cases the insertion depth was approximately 60% of the needle length and the height reduction after insertion was in both cases approximately 3%.
In both cases the insertion was non-random and targeted a specific locus.
In both cases, the insertion of a PAC is mandatory; because the rate VSD reopening after both procedures is considerable, the PAC is a potent diagnostic tool.
However, in both cases, the insertion of DNA was potentially the trigger for the emergence of novel CGI forming the gDMR in this locus.
The TF2497 and TF2834 strains have gene-trap proviruses in the Usp4 and Usp15 genes respectively; in both cases the insertion disrupts the gene near the 5′ end and precludes expression of a functional gene product (indeed no transcript can be detected by the sensitive method of reverse-transcription/polymerase chain reaction).
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However, in some cases the insertion sites were slightly altered, which indicates that they have once left and then been reinserted in the same position.
In both cases, the genomic regions surrounding the transgene insertion sites contain interspersed low complexity repeats, particularly LINE elements.
A second intervention, a so-called top hat, is already on the seabed, waiting to be maneuvered over the biggest leak in case the insertion pipe fails.
During the management of the last case, the insertion of the Blakemore® tube was controlled by using ultrasonography.
In an interference-loaded case the insertion loss of the switches is less critical as SINR is largely determined by co-channel interference at the antenna elements.
In this case the insertion loss of the switches, which affects signal and interference equally, has limited effect on receiver SINR, making net gains easily achievable.
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