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Two spiked bottles contained a yeast for which a PNA-FISH probe was not available (C. pelliculosa, Cr. neoformans); both bottles were negative with the yeast dual probe and Traffic Light probe [Figure 1].
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The remaining aerobic bottles were negative after prolonged incubation (up to 28 days).
All areas of the dairy processing plant not in contact with contaminated cheeses or spray culture solution bottles were negative for L.m.
A plastic bottle is negative for the environment.
The positive predictive value for sepsis was 18% when one bottle was positive and 37% when both bottles were positive.
When both bottles were positive, the bottles inoculated with 10 ml of blood showed growth sooner (P < 0.001).
Both bottles were stored in cold and dark conditions for posterior filtration.
Liquids in both bottles are transparent.
Sub-culturing BACTEC™ bottles which were negative after 14-days' incubation detected only a single additional Propionibacterium isolate from 1000 bottles.
Both tests were negative.
Both controls were negative.
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CEO of Professional Science Editing for Scientists @ prosciediting.com