Unlike the prostate epithelial cell line response, both benign and malignant primary prostate epithelial cells did not induce significantly more endothelial invasion in the presence of a BMS stimulus than the no prostate epithelial cells control (P=0.8233 and 0.2208 for CaP and BPH, respectively).
CNS tumours were defined according to main group III of the 3rd edition of the International Classification of Childhood Cancer (Steliarova-Foucher et al, 2005), which includes both benign and malignant primary tumours of the CNS, but excludes germ cell tumours and lymphomas located in the CNS.
Using the same markers we had previously used to isolate the normal and malignant prostate hierarchy (Collins et al, 2001; Richardson et al, 2004; Collins et al, 2005), we show here that the most undifferentiated cells in both benign and malignant primary cultures are more resistant to irradiation.
For the rest, i.e., benign and primary malignant neoplasms, histologic confirmation is necessary.
In contrast, both datasets displayed significantly decreased SPRY2 expression, with metastatic tissues having significantly suppressed expression compared with benign and primary carcinoma) and with normal tissue), respectively.
Primary lesions consist of both benign and malignant lesions.
Furthermore, amplification of the number of androgen receptors can be shown in hormone refractory tumours, when compared to both benign prostatic hyperplasia and primary prostatic malignancy, using both reverse transcription PCR and fluorescence in situ hybridisation (Bubendorf et al, 1999; Linja et al, 2001).
Next we used immunocytochemistry to study NM23 protein in normal skin, benign naevi and primary and metastatic melanomas.
Thus, it may be speculated that benign nevi and primary and metastatic melanomas express different BRMS1 variants with different biological functions.
To compare ASPM protein levels in established cancer cell lines, benign samples and primary cultures, a slot blotting technique was applied.
