Effective alignment free approaches should allow us to address such issues, particularly through the development of properly grounded regression approaches to protein sequence analysis.
Going forward, computational approaches should allow for the detection of novel potential drivers based on the mutability of repeats with proximal repeats.
Notwithstanding possible side-effects on the development of the field, such a large dataset of species-level microevolutionary approaches should allow addressing important issues in comparative biology.
To promote such insight, treatment approaches should allow patients to take responsibility for themselves rather than being highly regulated and therapist-controlled [ 26, 29].
Future studies with targeted metabolomic approaches should allow us to measure the degree of overlap (or lack thereof) for metabolites that show diet- and age-specific changes in correlation structure.
The use of single-cell and more quantitative approaches should allow us to elucidate whether the cell cycle-associated variability in response to TNF is cell type-specific or whether there is a generalizable effect.
In principle, comparative proteomic approaches should allow researchers to identify phagosomal proteins that are conserved in different species and to track changes in the protein content during phagosome maturation [ 52].
Although in vitro cloning approaches based on the use of high-fidelity polymerases for PCR amplification have significantly improved in recent years, the use of in vivo approaches should allow a more accurate method of mutagenesis due to the use of the cells own high-fidelity replication system which includes proof reading.
Such approaches should allow the field to move beyond correlations between various anatomical and physiological disturbances in mutant animals to direct tests of how the precise connectivity patterns and strengths between different cell types affect the information-processing parameters of microcircuits and larger neuronal networks and impact on specific behaviors [ 116, 118, 154, 162].
The improvement of RNAi in conjunction with proteomics and other approaches should allow us to characterize the effects of sortilin inactivation on the sorting of soluble lysosomal proteins other than SAPs and to determine if sortilin is responsible of a more general mechanism of lysosomal sorting.
