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To molecularly test this hypothesis we generated marked clones for dSnoEx17B or dSnosh1402 in third instar larval wing disks.Results with both alleles were consistent and those of dSnoEx17B are shown.
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In total, 98.19% of genotyping alleles were consistent with each other.
These alleles were consistent with known HLA alleles previously assigned by the conventional methods (Additional file 4: Table S3).
In each case, 7030 alleles were consistent with the parents and no additional bands were detected (data not shown).
At a more detailed level, patterns of DNA methylation at specific CpGs between carriers and non-carriers of risk alleles were consistent with previous studies.
All of the genotypes and linkages of DRB1 and DRB3/4/5 alleles were consistent with known alleles (Additional file 3: Table S2) and previous publications [ 32, 37], suggesting that a combination of our multiplex PCR methods and the Ion PGM system is an efficient and accurate HLA genotyping method for the detection of HLA alleles to the field 3 level of genotyping without phase ambiguity.
However, since NC1 homozygotes show slightly higher incidence of young adult heart failure than outcrossed heterozygotes (61% versus 52%, Table 1), the observed fraction of 73% NC1 alleles is consistent with the expected segregation ratio if the 97C allele accounts for the majority of the genetic defect.
The distribution of alleles was consistent with that found in the Caucasian population.
Indeed, the population frequencies of many of the P element bearing heat-shock alleles is consistent with positive selection [2], [15].
All data from sequencing of bulk PCR products and cloning of individual PCR-amplified alleles are consistent with this SNP representing a sub-variant of SPRNa.
The overall prevalence of ABO types, and of FUT2 (secretor) and FUT 3 (Lewis) alleles was consistent with that found in the Caucasian population.
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