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Both alignments can be downloaded from our webpage.

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Therefore, GSNAP will favor concordant solutions involving suboptimal alignments on one or both ends, even when better alignments can be found individually for each end.

Both spliced alignments and local alignments can be used to detect novel transcriptional and splicing events including exon boundaries, exon-exon junctions, gene boundaries, transcriptional start (TSS) and transcription end sites (TES).

These alignments can be detected even when the DNA sequence is too divergent to align easily.

Moreover, alignments can be further investigated, by attaching GO terms to the proteins of aligning networks.

Such alignments can be used for similarity-based identification of new compounds by using known active template structures as seeds.

Multiple sequence alignments can be used to create a phylogenetic tree.

Since alignments can be ranked, correct assignments will be saved as unique alignments in the first filtering step (Figure S1).

These alignments can be downloaded from our website.

Therefore obtaining valid multiple sequence alignments can be quite challenging.

The alignments can be further used to detect conserved motifs.

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