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This variation confirms that both AGO fusion proteins are concentrated at many small foci.
In the ovary both AGO proteins are predominantly cytoplasmic, with much lower levels in the nuclei.
A common characteristic of all types of small RNAs is their association with proteins of the ARGONAUTE family, which is comprised of both AGO and PIWI members [6], [7].
Members of both AGO and Piwi subfamilies possess the characteristic DDH metal binding signature residues in their Piwi domains, while most of the WAGO proteins lack them.
Electron microscopy imaging revealed enrichment of atAGO1 in proximity to the Golgi apparatus (Derrien et al, 2012), reminiscent of the situation in animal cells where both AGO and Dicer localize and fractionate with membranes of the Golgi apparatus (Cikaluk et al, 1999; Tahbaz et al, 2004).
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Using this procedure we were able to achieve robust reductions (>80%) in both ago-2 and vlg-3 mRNA levels, as measured by quantitative real-time PCR (qPCR).
Phylogenetic analyses suggest that both Ago-like and Piwi-like proteins were present in the last common ancestor of eukaryotes, but one or both have since been lost in specific lineages [ 15].
Yet, only a few decades ago, both the Santorum candidacy and the Ryan nomination would have been unthinkable.
Not long ago both the rubber-band chair and Mr. Chamberlain briefly caught fire when welding sparks shot the wrong way.
Since Soriano shifted to second base last year and Hillenbrand became a third baseman less than two years ago, both also had to adapt to new positions.
When flowering plants took over about 100 million years ago, both the kalligrammatids and the plants they fed on went extinct.
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