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Thus, the incorporation of a linker and antifouling molecules is an important interfacial design for both affinity and enzymatic biosensors.
Among all the possible selection pathways, we observed improvements in both affinity and crossreactivity only when recombinant genetic libraries were screened in order from lowest to highest affinity chemokines (Fig. 2b, d).
Moreover, by comparing the binding characteristics of the two proteins at varying salt concentrations, we show that the ionic strength makes significant and variable contributions to both affinity and specificity.
While alkyl and benzyl substitution provided affinities and antagonist potencies comparable to those of 4-PIOL, diphenylalkyl and naphthylalkyl substitution resulted in marked increase in both affinity and potency.
We were interested in assessing the effect of this substitution pattern on both affinity and intrinsic activity, as the dicyanopyridines comprised both agonists and inverse agonists, whereas the original aminopyrimidines were exclusively inverse agonists.
A particularity of carbohydrate protein recognition of lectins is that both affinity and specificity are achieved due to polyvalent interaction, whereas affinity of single ligand to lectin can be very low or not registered at all.
Similar(29)
NRT1.1 (also known as CHL1 and NPF6.3), which belongs to NRT/PTR family, functions in nitrate uptake as both high affinity and low affinity transporter23.
The classical D2DR agonists were able to negatively modulate both A2AAR affinity and functionality.
Additionally, we review experiments on effects of P, N and N + P addition on both low affinity and high affinity methane oxidation.
The goal of designed PPIs is to achieve both high affinity and specificity for the target protein.
Modifications to these structures were assessed in silico for their influence on both 5HT1A affinity and metabolism.
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