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Both adapter and test stimulus RDKs were presented within a circular area with a diameter of 14°.
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Note that there was no spatial overlap between adapter and test stimulus.
We quantified the subjective magnitude of the MAE induced by two different delay durations between adapter and test stimulus (50 and 500 ms).
In the third panel, adapter and test stimulus are on the same side but with a delay of 500 ms between the two.
This positive control condition is closest to the storage condition of the first experiment with the difference of an intervening saccade between presentation of adapter and test stimulus.
In the fourth panel, adapter and test stimulus were on opposite sides and were presented with a delay of 500 ms. The test stimulus was shown after the saccade and at the same retinal coordinates where the adapter was displayed.
In the second case, when adapter and test stimulus are on opposite sides and the delay between the two is 50 ms, the saccade is being prepared at the same time the test stimulus is shown.
In the first case, when adapter and test stimulus are on one side and the delay between the two is 50 ms, a saccade is being prepared at the very moment the test stimulus is shown, whilst the subject's eyes are still stationary.
dsDNA carrying both adapter A and adapter B attached to its ends was selected and amplified with PCR using a proof reading enzyme (24 cycles).
The tags with both adapter 1 and adapter 2 were then prepared for Illumina DNA sequencing [ 35].
Both sequencing adapter and cDNA synthesis primers were removed in an additional quality filtering step.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com