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Exact(2)
In this substrate, both activity and transcript increased with approximately the same rate during the culture period.
We note here that the unchanged amount of malate was surprising because it has been shown that addition of malate reduces both activity and transcript level of nitrate reductase [ 29].
Similar(58)
More in detail both enzyme activity and transcript level of phenylalanine ammonia-lyase (PAL), a crucial enzyme involved in the biosynthesis of phenolic metabolites of the phenylpropanoid class, comprising hydroxycinnamic acid (HCA) derivatives, lignans and flavonoids [ 61], were found as strongly induced in inoculated tissue at the same time-point [ 60].
However, it is not clear whether that was due to species-specific differences in promoter activity and transcript fidelity without the influence of transposon integration site effects.
By contrast, the correlations between activity and transcript levels of the other enzymes appeared to largely follow the same trends in both strains (Additional file 5: Figure S3B).
However, it is known that there may not be direct correlation between enzyme activity and transcript abundance.
Together, the enzymatic activity and transcript data suggest that the CYP4BL3 protein may be responsible for the bulk of the observed eicosanoid hydroxylase activity in Cyphoma digestive gland.
Sucrose degradation, and glycolic- and fermentation-related enzyme activity and transcript levels by qRT-PCR were analyzed after HAT and 3 days later.
Interestingly, Cys pool was significantly higher in TM plants than MC, MuC, and MuT despite nonsignificant change in SAT and OAS-TL activity and transcript abundance.
The discussed changes in the correlations between activity and transcript abundance were statistically significant for all enzymes with the exception of Pdc.
Trypsin protease inhibitor (TPI) activity and transcripts in transformed lines; graphs show means+SEM.
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