Sentence examples for both active and mutant from inspiring English sources
Smooth muscle cells were then cultured in conditioned medium from both active and mutant caspase-1 transfected cells.
E. coli DH5 α were transformed with both constitutively active and mutant PKC α using heat shock, and selected with ampicillin (100 μg ml−1).
We have shown that AR149 and both (active and inactive) ARF6 mutants partially colocalize on endocytic vesicles and the plasma membrane (Figure S7).
Constitutively active and mutant plasmids of PKC α were a gift from Prof. Peter Parker (Cancer Research, UK).
Both, the active and inactive mutant heparanase were ∼30 fold over-expressed in the Hpse-transfected cells as compared to mock transfected cells (Fig. 8A).
Complementing the skn7 ∆ mutation with both the active and inactive mutant forms of Skn7p led to rescue of the mat formation defect of skn7 ∆.
NtRabA (Rab11) in tobacco is predominantly localized to an inverted cone-shaped region at the pollen tube tip, and both constitutively active and dominant negative mutants resulted in reduced tube growth rate, meandering pollen tubes, and reduced male fertility [ 20].
Active and inactive mutants of MTI-2 are constructed and displayed on phage.
Conversely, reinsertion of CASP2 (both normal and active site mutant: cysteine [C303] is mutated to alanine) inhibited autophagy, confirming a role for CASP2 as a negative regulator of autophagy, which was independent of the presence of the catalytic active site (C303) (Fig. 2C and D).
Both mutants were less active and displayed 84% (V246M mutant) and 15% (G385F) KDS production relative to the wild-type SPT enzyme.
A series of studies using both expression of active and dominant-negative Rit mutants and RNAi-mediated gene silencing approaches had identified putative roles for Rit in the control of signaling pathways involved in neuronal morphogenesis,, differentiation - and cell survival., However, whether any of these functions constitute an essential cellular role for Rit remained uncharacterized.
