Interestingly actin and talin clearance from the synapse were also impaired in Lck-deficient CTL.
Fluorescence expression of both the actin cytoskeleton and talin was significantly increased.
Mutant cells deficient in both talin A and talin B, actin-binding proteins involved in cell-substratum adhesion, also cannot attach to the substratum (Tsujioka et al., 2008).
Both Src and talin were identified in our study.
In addition to vinculin and actin, talin recruits other integrin-associated proteins [ 12], providing a scaffold for protein complex assembly.
Acquisition was performed with an Olympus BH2-RFCA epifluorescence microscope (Olympus, Japan), equipped with a 100W mercury lamp (OSRAM, GmbH), using a SPlan achromat x 40 objective (Olympus) and fluorescein, rhodamin, DAPI filters for talin, actin and nuclei staining, respectively.
Furthermore, the down-regulation of talins (actin and integrin binding proteins) may explain per se the misalignment and apparent failure of adhesion observed in the mATX3-depleted cells given their key role in focal adhesions and cell spreading [52].
The NMR structure of the FABD (aa-998 to aa-1130) shows a four-helix bundle (Figure 6B), which is also found in several other F-actin binding proteins such as vinculin and talin [38], [39].
Vinculin has binding sites for actin and other proteins such as talin, α-actinin, VASP, Arp2/3, vinexin, nArgBP2 and CAP which can all associate with the actin cytoskeleton.
