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However, when possible, both actin and cyclophilin B levels were assessed and the levels of both proteins were similar in each group (Fig. 6F,G).
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Both actin and myosin constitute the cytoskeletal architecture.
Both actin and ARPs bind ATP.
Cdc42EP3 can bind both actin and septins and localizes between F-actin fibers and SEPT2 filaments.
Knockdown effects were normalized to the housekeeping genes β-actin and cyclophilin B, respectively.
Threshold cycle numbers during the log-phase of amplifications were normalized to the expression of β-actin and cyclophilin.
Two of the housekeeping genes (beta-Actin and Cyclophilin A) showed a marked difference between the two cell lines and the two time points.
The β-actin and cyclophilin primers also produce larger PCR products from genomic DNA and therefore could also be used to assess the amount of contaminating DNA in the cDNA preparation.
Relative transcript levels were calculated using the ΔΔ-ct method for the two housekeeping genes β-actin and cyclophilin B. Means, p-values and confidence intervals were calculated using the software package SPSS.
We also included two internal control transcripts (vascular α-actin and cyclophilin B) that are also expressed in the uterus/ovaries of the immature rat but whose expression is not regulated by estrogens in these organs.
The results for GLUT-1 were normalized using two housekeeping genes, β-actin and cyclophilin A, determined with Genex® software (MultiD Analyses AB, Göteborg, Sweden) as the most stable under the experimental conditions used.
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