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In the course of constructing our expression vector, we generated both a low copy and high copy version of our expression plasmid, by addition of a single base pair mutation in the origin of replication.
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Interestingly, subfamily A has a low copy number in both GIII and Oregon-R, suggesting the recent birth of this subfamily or its low transposition efficiency.
The growth defect and the acid resistance defect of the tolC strain were both complemented with a complete tolC gene on a low copy plasmid.
Therefore, we sequenced the full genome of W. chondrophila using both 454/Roche and Solexa/Illumina technologies, also uncovering the presence of a low copy number plasmid.
There can be multiple mechanisms by which a copy-up mutant can outcompete a low copy wild type.
A low copy number is optimal for long term survival of the plasmid in a host cell lineage.
In general gypsy shows a low copy number in the A (X) chromosome compared to autosomes.
Some viral genomes adapt to a low copy number balance in stem cells and avoid detection.
This mouse line has a low copy number of the human MAPT transgene.
The third haplotype detected in the ITS-1 was represented in a low copy number (2.06%).
The cDNA fragments amplified from the genome of DHAV-3 were assembled and inserted into a low-copy-number plasmid.
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