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While GLI3 is predominantly a transcriptional repressor, GLI2 exists in both a full length active form and a truncated repressor form.
You will need both a full length mirror and a handheld mirror.
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For this reason, we queried the organism sequence databases with both A) full-length type I and II human cadherins, and B) their cytoplasmic tails alone.
Using nomenclature assigned by Ragon et al. [ 24], isolates 08-5578 and 08-5923 both encode a full length inlA allele type 2 that has previously been observed in isolates related to human illness [ 24].
This was a surprising result since the NLS had been shown to cause localization to the nucleus in the context of both a smaller fragment and a full length construct containing a kinase defective mutation in a different cellular context (Perander et al., 2001).
Look for a full length bar.
Cytoplasmic localization of the huntingtin protein has previously been shown to be sensitive to leptomycin B both as a full-length fluorescent protein fusion (15) and in the context of endogenous huntingtin (38, 39).
All these genes can code for both a full-length protein and an isoform without the PR-domain, which is often overexpressed in cancer [ 22, 23].
We note that in these experiments we consistently detected both a full-length histone H3 band and a significant degradation product.
Similar to p63, it is also expressed as both a full-length TAp73 (or p73) and a dominant-negative truncated form ΔNp73.
The study was conducted on the 54 genes in which both a full-length EST contig and a full-length genomic sequence could be established for the entire open reading frame (see Additional file 4: Figure S3).
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