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The effects of both TNFRs were very similar and the information in them redundant.
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In situ hybridization corroborated RTqPCR analyses and, additionally, showed that both TNFRs were ubiquitously expressed in zebrafish embryos (supplementary material Fig. S2B).
This indicates that an appropriate signaling balance between both TNFRs is required for endothelial cell integrity and vascular homeostasis.
However, the immunoreactivity of both TNFRs was less intense in carcinoma cells than that in epithelial cells in cervical specimens with non-specific inflammatory changes.
Gene expression analysis by quantitative real-time reverse transcriptase polymerase chain reaction (RT-qPCR) showed that the mRNA of both TNFRs was present in zebrafish embryos at spawning, indicating that it was maternally transferred (supplementary material Fig. S2A).
Furthermore, the simultaneous depletion of TNFRSF1A or the activation of NF-κB rescue endothelial cell apoptosis, indicating that a signaling balance between both TNFRs is required for endothelial cell integrity.
Both men were very shy.
"Both goals were very well played.
Both were very good.
"Both my parents were very supportive.
"Both were very supportive to me".
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