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Cultures were grown in a BioSafety Level 3 laboratory at Grenoble University Hospital because results of both PCRs were positive.
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All three PCRs were positive for 57 (56 EHOS) isolates, and these were considered HPI-positive.
In 1 case, both BALF Grocott's staining and PCR were positive for Pneumocystis jirovecii.
Both acute-phase serologic tests and serum RT-PCR were positive for SARS-CoV (National Microbiology Laboratory, Health Canada, Toronto).
†Taken as positive only when both upstream of E gene and open reading frame 1a RT-PCR were positive.
Among the clinical samples, all samples that tested positive by conventional PCR were positive by SYBR Green real-time PCR (data not shown).
Two of the 8 samples assayed by nested PCR were positive and 5 of 12 samples assayed by quantitative PCR were positive.
CSF culture and PCR were positive in 60% and 90% of patients, respectively.
A sample was considered positive when the qRT-PCRs were positive for the 2 different specific genes.
In this patient, qualitative PCR was positive in both urine and peripheral blood.
Regular PCR targeting the intertransgenic spacer (ITS) was negative for this brain biopsy; however, repeat PCR was positive for both the biopsy specimen and cerebrospinal fluids.
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