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According to this molecular marker profile, we identified these cells as recently born GCs.
We reasoned that if PROS could arrest proliferation in new born GCs, it might do so by regulating dap expression in these cells.
For the shake of simplicity we have called them A and B. In type A, PROS is expressed in GCs after the division of GMCs while in type B, PROS is first expressed at low level in the NB and asymmetrically segregated to the GMC, and afterwards, upregulated in new born GCs.
Similar to developmentally born GCs, we find that adult-born GCs employ Sema5A to negatively regulate dendritic spine density.
One family of extracellular cues known to regulate the morphology of developmentally born GCs is the semaphorins.
5) By the way the Bcl2 experiments are presented and discussed, it remains unclear whether spine formation is required for the survival of adult born GCs.
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The transient upregulation of pros mRNA as well as the high levels of PROS protein in newly born neurons (GCs) suggested a possible novel role for PROS in the generation of postmitotic cells during postembryonic neurogenesis.
This shows that Sema5A negatively regulates dendritic spine development in adult-born GCs in vivo.
(H – K ) Recordings of spontaneous mEPSCs from retrovirally-labeled adult-born GCs at 19 21 days postmitosis.
10.7554/eLife.04390.007 Figure 2. In adult-born GCs, Sema5A negatively regulates dendritic spine density and affects their electrophysiological properties.
In addition, the loss of Sema5A increases the amplitude of AMPA receptor currents in these adult-born GCs.
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