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After elution, the borders of the plates were revealed with solution of vanillin-sulfuric acid (1% vanillin and 1% H2SO4 in ethanol) for visualization of the metabolites and its further isolation.
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At higher resolution, the images show quasi-spherical grains in the border of the plates which appear to coalesce forming the lamellae (Figure 3b).
Lines were drawn with a waterproof marker from the screw heads to the plate surface and additional lines were drawn on the bones along the border of the plates.
This material, when not surrounding a specific defect spot, is concentrated on the exterior border of the plate, a region prone to tarnish from contact with the metal frame of the daguerreotype plate.
When the fungal mycelium reached the border of the plate (approx. 14 days), three untreated and three modified mini-blocks were aseptically added on separate metal grids (to avoid moisture uptake by the wood block).
Antimicrobial activity against fungal/oomycete pathogens was determined by spotting suspension of washed cells (see above) on R2A, TSA, PDA, 1/5th strength PDA or 1/10th strength TSA medium at the border of the plate and inoculating a plug of the fungus or oomycete in the middle.
The CapI-lox5 transcript is first detected at stage 5 in large ventro lateral ectodermal domains, extending to the ventral and posterior borders of the belly plates on both sides of the ventral midline (Figure 8A and 8B).
The specific enrichment of apoptotic cells observed at the lateral borders of the neural plate of PGK Cre Dbx1DTA embryos could thus preclude fusion of the neural folds.
In UALVP 47273, the neural canal seems to end at approximately the anterior border of the minor plates that comprise the distal end of the knob (Fig. 4).
CapI-Dfd expression is first detected on both sides of the ventral midline along the medial border of the belly plates in the presumptive VNC at early stage 5 (Figure 6A and 6B).
CapI-Scr expression is initiated as the first segments form in bilateral domains at the medial border of the belly plates in T3 and T4 at early stage 5, with weaker expression in the ventro lateral ectoderm of these segments (Figure 7A).
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