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One month after surgery, only the borders of the patch were reendothelialized), whereas, in the other areas, several platelets adhered to the exposed collagen fibers).
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Note that the sharp borders of the patches dissolve with increasing diffusion, and the pattern of the arena becomes "normal", i.e., nearly homogeneous.
Overland flow at the inter-patch was attenuated as it reached the border of the patch, diverging from the latter as it followed the (small) topographic gradient.
In summary, we found that (1) profiles of the classification images for perceived brightness peak at the border of the patch.
Classification images for three subjects are presented in figure 5. Classification images reveals positive peaks inside the border of the patch and less prominent negative peaks in the background immediately next to the border.
Results ("raw" and "normalized" classification images) are shown in figure 4. Classification images peak inside the border of the patch and have negative peaks in the background, next to the border.
The border of the patch is approximated by the convex hull of the interest points.
These patches could be distinguished from the connective tissue monolayer by a light birefringence on the border of the patches (Fig. 2D,E; arrows).
In a 15 cm diameter glass Petri dish, 16 pieces of wheat leaf (separated by 0.5 cm) were placed within a 9 cm diameter circle drawn with red ink that was considered the border of the experimental patch.
As postulated, Snf7-Atto647N fluorescence appeared only at the border of the growing patches.
After delineating the borders of immunoreactive patches, their width was measured using ImageJ and compared using Student's t-test (GraphPad Prism version 4 for Windows).
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