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RM5448 and RM5310 represented the outside borders of the introgression.
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The pattern of introgression, most pronounced near the borders of the Cariaco drainage, suggests the role of natural processes and not of human-induced introductions.
Fig. 1 Frequency distribution of the introgression segments per each IL.
Together, these data strongly suggested that, under salt stress, the introgression increased the Na+ content in shoots of of the introgression line via down-regulation of OsSOS1, OsCIPK24, OsCBL4 and OsHKT1 5.
To validate whether the resistance of the introgression lines was associated with the Pi2/9 haplotypes, each BC3F2 population used for the advancement of BC3F3 introgression lines was proceeded with genetic analysis.
The process of the introgression is outlined in Figure 1.
Only field testing of interspecific introgression lines would assess the agronomic and breeding values of the introgression lines.
In the scenario involving several introgression events, part of the introgression occurs later than in the scenario with a single introgression event and, thus, the exogenous material is easier to remove.
The reasoning is as follows: T. macedonicus newts that colonized the northwestern part of the introgression zone derived from the stock in the south of the introgression zone.
The borders of the resulting clones were sequenced using universal primers (M13R for BamHI borders and M13F for XhoI borders).
The boundaries of the introgressions were established and the source of several introgressions was determined.
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