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The borders of the defects were identified by the interfaces between presumed original cartilage and newly formed fibrocartilaginous/fibrous repair tissue on both sides.
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Once the bony borders of the defect were identified the surrounding mucosa was elevated.
The nasoseptal flap covered the bony borders of the defect and was fixed either with blood and Surgicel © or with fibrin glue, the latter not being mandatory.
Although the control tissue-engineered cartilage showed some tissue repair along the borders of the defect, the color of the tissue was slightly different from that of the surrounding normal cartilage.
When left untreated, the major tissue in the negative control samples was dense connective tissue and bone formation was limited to the border of the defect.
This safeguard enabled the exact identification of the circumferential and superior borders of the cylindrical defects during tissue processing and analysis.
The rectangular ROI, which encompassed the entire defect, was delineated from the adjacent bone by the clearly identifiable borders of the drill defect.
To judge its benefit, it is important to differentiate between bone formation induced by cells from the border of the osseous defect (osteoconduction) in relation to bone matrix produced by the implanted cells (osteogenesis).
Secondly, the increased perfusion near the lateral border translated into a gap between the border of the perfusion defect and the border of the infarct, as previously described.
It's one of the defects listed.
The borders of the resulting clones were sequenced using universal primers (M13R for BamHI borders and M13F for XhoI borders).
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