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Imaging software CellD (Olympus) was used to circumscribe the borders of each plaque to generate plaque area (μm) as described previously [ 18, 19].
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The outline of each plaque was marked manually on still images, with calculation of plaque area.
The borders of each of these entities were different.
Blue rectangles are located at the borders of each TAD.
The borders of each individual OHC were outlined based on the phalloidin staining.
The ssearch3 program was also used to define the borders of each repetitive sequence.
In order to evaluate the spatial distribution of Arg I and Arg II labeling within the plaque, we have set up a morphometric method based on intensity profiles of each immunostain and a normalization of the distance between the internal elastic lamina (IEL) and the luminal border of the plaque.
α-SMA immunoreactivity was located along the intima-luminal border of the plaque.
Moreover, it is abundantly expressed within the subendothelial regions and in the intima-media layers at the border of atherosclerotic plaque.
As NfH reaches the border of the acute plaque and in the spinal cord it becomes hyperphosphorylated.
The lateral borders of the ROI were removed to exclude artifacts on the border of each image.
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