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Sensory proliferation initiates in narrow zones along the proximal and distal borders of each annulus; these zones rapidly expand to fill the mid-annular regions.
Mitotic proliferation of sensory cells initiated about 20 24 hrs after pupation (a.p ., in pre-existing zones of high density cells lining the proximal and distal borders of each annulus.
In our study, mitotic activity was observed to initiate within the presumptive olfactory epithelium along the proximal and distal borders of each annulus and to expand inward into the mid-annular regions.
In both non-diapausing and diapausing animals, the presumptive sensory epithelium initiates development, after pupation, with two zones of high density cells lining the proximal and distal borders of each annulus and a large field of low density cells filling the middle annular regions.
High density zones of small cells line the proximal and distal borders of each annulus within the presumptive sensory epithelium, observable as early as 2 hrs a.p.; the distal zone is consistently broader than the proximal zone and is discontinuous at the longitudinal mid-line (see Fig. 3A ).
Another possible exception is the zones of high density cells lining the proximal-distal borders of each annulus; these cells were observed as early as 2 hrs a.p., and if these cells derived from mitotic events, those events must also have occurred earlier than 12 hrs before pupation.
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In males (A) and females (B), BrdU incorporation initiated in two narrow zones, one along the proximal and one along the distal border of each annulus.
BrdU incorporation followed a temporal-spatial progression, initiating along the proximal and distal borders of each presumptive annulus and then expanding into the mid-annular region ( Fig. 2A,B ).
Using TDI, the peak early diastolic myocardial velocities at septal and lateral borders of the mitral annulus were measured and averaged to calculate the mean early diastolic myocardial velocities (E′).
Annuli of various sizes were used to mask portions of the grating (Fig. S8A); luminance of each annulus was set to 128 (0 and 255 for black and white, respectively).
When used to create a radial profile, the output now contains the RMID column, which gives the mid-point of each annulus in pixels.
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