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In addition to common tagSNPs, we selected the following variants for follow-up genotyping: (i) variants located in exons or intron exon junctions, (ii) uncommon/rare variants (MAF<0.05) present in ≥2 individuals included in sequencing and (iii) suspicious (borderline quality) novel variants identified in sequencing.

When 'no-call' procedure is applied to those observations of borderline quality, we showed earlier that the biased estimation is dictated only by MAF and c.

As for the less than stellar features, the inclusion of a 2.5mm headphone jack is just plain inexcusable, especially since the phone is marketed as a "V Cast Music Phone with Rhapsody," all but limiting its musical enjoyment to crappy borderline quality stereo Bluetooth headphones or wonky 2.5mm cellphone headsets.

The commonly used 'no-call' procedure for the observations of borderline quality should be modified.

One additional partial failure occurred in a sample with borderline quality parameters.

The commonly used 'no-call' procedure does not always a best option for observations of borderline quality.

The first option is to discard the observations of borderline quality using the 'no-call' procedure which may lead to MCB.

As shown above, the commonly-used 'no-call' principal for the observations of borderline quality is not always the best choice.

Otherwise, it is better to call the observations of borderline quality at the cost of genotyping errors (grey area above the line in Fig. 4B, and Additional file 4B & 5B).

It was suggested that 'no-call' procedure should be taken where observations of borderline quality be removed from allele calls in order to keep the genotyping error rate as low as possible [ 29].

We propose that although BPD patients can have concomitant MD, they often exhibit a specific BPD-associated affective syndrome that is different from both MD and non-borderline RBD in the quality and duration of symptoms and the biological substrate.

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