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In the Cation efflux and Zip families, a domain border was determined using identification of ~100 residue length alignment gaps concurring with low complexity regions in the dominant Pfam architecture [Table 6].
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The boundary of CNVs was determined using 90% density borders [ 20].
The location of the mediastinal ROI was determined using the following anatomical landmarks: the lung apex as upper border, the upper cardiac border and the medial contours of the lungs (Fig. 1).
Tumour size was determined using calipers.
Peptide binding was determined using ELISA.
Iodine uptake was determined using radioactive iodine.
Signal intensity was determined using GeneChip Operating Software 1.4.
Proteasome activity was determined using fluorescence assays.
Survival was determined using Kaplan Meier test.
Protein concentration was determined using a BCA Assay Kit Piercee).
Differences in the proportions of labile chromosomal features in amplification patterns and within border bands or inside amplification patterns were determined using a hypothesis test (Table 1).
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