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The left border sequence of T-DNA was completely deleted from the vector and not integrated into the genome of alfalfa in the transgenic plant B196.
Transformants are readily obtained in which tandem copies of the vehicle plus insert are inserted between the two genomic border sequence homologues.
During the process of T-DNA transfer from Agrobacterium tumefaciens to plants, T-strand synthesis typically initiates at the right border sequence but can terminate short of, at or beyond the left border element [31].
Several different types of border sequence arrangements were detected.
A portion (25%) of the remaining lines contained a second left border sequence or internal T-DNA vector sequence which identified a nested integration event.
The expectation was that each FST sequence would contain the T-DNA left border sequence and the adjacent A. thaliana genomic sequence.
Similar(49)
Except in cases where the recipient genome was obtained with a short vector sequence, truncation of the T-DNA termini occurred in 89.5% (17/19) and 15.8% (3/19) of the border sequences on the left and right borders, respectively.
Previous characterisation of the balhimycin biosynthetic cluster had shown that the border sequences contained both, a putative 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (dahp), and a prephenate dehydrogenase (pdh) gene.
Progeny of a transformant are subsequently screened for those with a decreased resistance to the antibiotic, and then for those which have completely lost the marker and the entire vehicle, leaving only the desired sequence modifications between the two genomic border sequences which were targeted.
Xca and the Xcc strains share the same left and right border sequences of Regions I and II, again reflecting the close relationship between these two pathovars.
These results are included in Table 4. Gene-specific primers were used in combination with T-DNA border sequences to confirm insertion sites.
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