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Nevertheless, all three enhancer trap lines share the same anterior border of expression in PS5.
Meanwhile, the anterior border of expression of the next three enhancer trap lines (within the region colored in red) is shifted one parasegment posterior, in PS6.
Notably, the directly adjacent epithelium is likewise marked by expression of three cluster A ESTs showing a precise border of expression between the ESTs.
If we focus on the three transposons inserted within the 75 kb region colored in orange, we find that the anterior border of expression of the lacZ reporter genes marks precisely PS5.
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The existence of crypto-rhombomeres was subsequently corroborated by the observation of corresponding molecular limits, namely step-like arrangement of the rostral borders of expression of Hox genes of the 4 8 paralogous groups, analogously to the patterns of paralogous Hox gene groups 1 3 across the overt rhombomeres (Marín et al. 2008).
We further show that the border of RhAG expression domain is labile, which allows the selection of rose flowers with increased petal number.
Green arrowheads mark the anterior border of sim expression.
Second, the anterior border of lacZ expression always progress toward the posterior by increment of one parasegment.
The red arrowheads mark the posterior border of msh expression which is positioned at approximately 60% egg length (0% posterior pole) in wt (A ) and tsg KD (D ) embryos.
It must ensure that within its own borders, freedom of expression and freedom of association are protected and defended.
We also emphasize that the enhancer analysis is focused on central regions of the knirps expression pattern, outside the limits of the repressive interactions that help establish the sharp borders of the expression "stripe".
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