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The bootstrap test was carried out on Nicotiana because it was the most numerous genus.
The unrooted phylogenetic trees were constructed with MEGA 4.0 using the Neighbor-Joining (NJ) method, and the bootstrap test was carried out with 1000 iterations.
The phylogenetic tree was constructed with the Molecular Evolution Genetic Analysis (MEGA) 5.0 software according to the fit model using the maximum-likelihood (ML) method and the bootstrap test was carried out with 1000 iterations.
Phylogenetic analysis was performed based on the full-length protein sequences using the MEGA 5 program by the NJ method, and a bootstrap test was carried out with 1000 interactions [ 84].
The protein sequences of Arabidopsis and rice auxin response factors were obtained from the TIGR database and phylogenetic analysis was performed with MRBAYES 3.1.2 program [ 68] by Bayesian method [ 69] and the bootstrap test was carried out with 1,000,000 iterations.
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The Poisson correction model and Neighbor-Joining method were used, and the phylogeny test was carried out by bootstrapping with 1,000 replications.
In addition, pairwise comparisons using the functional ANOVA method and a bootstrap distance based test are carried out to discern which factor levels provide different ways of degradation.
Six statistical tests were carried out simultaneously: Bootstrap Probability [ 41], Expected-Likelihood Weights (ELW) [ 42], Kishino-Hasegawa (KH) [ 43], Shimodaira-Hasegawa (SH), Weighted SH (WSH) [ 44] and Approximately Unbiased (AU) [ 45], all implemented in Treefinder [ 36].
One thousand permutation tests were carried out and the bootstrap histograms are shown in Figure 1.
Between 1946 and 1958, 67 tests were carried out.
All tests were carried out blindly.
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