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The MEGA5 program using the Neighbor-Joining method with default parameters besides 1000 bootstrap replications was used to generate the phylogenetic tree (Tamura et al. 2011), which was further edited by using the Interactive Tree of Life on line tool (iTOL, http://itol.embl.de) (Letunic and Bork 2016).
The bootstrap method with 100 bootstrap replications was used to calculate confidence intervals for the log titers [ 36, 37].
A non-parametric bootstrap approach [12] and the percentile method for 100 bootstrap replications was used to plot 95%% confidence regions for the screening sensitivity estimates.
The Maximum Likelihood method, based on the JTT matrix-based model [ 58] and with 1000 bootstrap replications, was used to generate a phylogenetic tree (Additional file 1: Figure S2).
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For each data set, 1000 bootstrap replications were used, resulting in a bootstrap percentile confidence interval for the AMA.
One thousand bootstrap replications were used to provide confidence in the nodes.
One thousand bootstrap replications were used to assess the degree of confidence to be placed in the nodes.
In the bootstrap estimation 1000 bootstrap replications are used to produce the bootstrap distribution of PR.
One thousand (1,000) bootstrap replications were used to evaluate the branched supporting values.
A total of 2,000 bootstrap replications were used to test of phylogeny.
For evaluation of cross-validation results, P values calculated from 50 parametric bootstrap replications were used.
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